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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TACC3 Polyclonal antibody (25697-1-AP)

    WB analysis using 25697-1-AP (same clone as 25697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25697-1-AP (TACC3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TACC3 Polyclonal antibody (25697-1-AP)

    IHC staining of human cervical cancer using 25697-1-AP (same clone as 25697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TACC3 Polyclonal antibody (25697-1-AP)

    IHC staining of human testis using 25697-1-AP (same clone as 25697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using TACC3 Polyclonal antibody (25697-1-AP)

    IHC staining of human testis using 25697-1-AP (same clone as 25697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using TACC3 Polyclonal antibody (25697-1-AP)

    IHC staining of human cervical cancer using 25697-1-AP (same clone as 25697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25697-1-AP (TACC3 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TACC3 Polyclonal antibody (25697-1-AP)

    IF Staining of HeLa using 25697-1-AP (same clone as 25697-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TACC3 antibody (25697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25697-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TACC3 Polyclonal antibody, PBS Only

TACC3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25697-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ERIC 1, ERIC1, ERIC-1, Maskin, TACC 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25697-1-PBS targets TACC3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22516

Product name: Recombinant human TACC3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 141-330 aa of BC111771

Sequence: SSESGPGALADLDCSSSSQSPGSSENQMVSPGKVSGSPEQAVEENLSSYSLDRRVTPASETLEDPCRTESQHKAETPHGAEEECKAETPHGAEEECRHGGVCAPAAVATSPPGAIPKEACGGAPLQGLPGEALGYPAGVGTPVPADSTQTLTCAHTSAPESTAPTNHLVAGRAMTLSPQEEVAAGQMASS

相同性解析による交差性が予測される生物種
Full Name transforming, acidic coiled-coil containing protein 3
Calculated molecular weight 838 aa, 91 kDa
Observed molecular weight160 kDa
GenBank accession numberBC111771
Gene Symbol TACC3
Gene ID (NCBI) 10460
RRIDAB_2880199
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6A5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TACC3, also known as ERIC 1, belongs to the TACC family. TACC family members TACC1, TACC2, and TACC3 map very closely to the corresponding FGFR1, FGFR2, FGFR3 genes on chromosomes 8, 10, and 4. Subsequently, since they are phylogenetically related, it is proposed that TACC and FGFR have similar roles in cell growth and differentiation. It plays a role in the microtubule-dependent coupling of the nucleus and the centrosome and involved in the processes that regulate centrosome-mediated interkinetic nuclear migration (INM) of neural progenitors.

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