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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using TACC2 Polyclonal antibody (11407-1-AP)

    WB analysis of mouse testis using 11407-1-AP (same clone as 11407-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11407-1-AP (TACC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11407-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TACC2 Polyclonal antibody (11407-1-AP)

    IHC staining of human lung cancer using 11407-1-AP (same clone as 11407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11407-1-AP (TACC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11407-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TACC2 Polyclonal antibody (11407-1-AP)

    IHC staining of human lung cancer using 11407-1-AP (same clone as 11407-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11407-1-AP (TACC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11407-1-PBS in a different storage buffer formulation.

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TACC2 Polyclonal antibody, PBS Only

TACC2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11407-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11407-1-PBS targets TACC2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TACC2 fusion protein Ag1923 相同性解析による交差性が予測される生物種
Full Name transforming, acidic coiled-coil containing protein 2
Calculated molecular weight 2948 aa, 309 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC015736
Gene Symbol TACC2
Gene ID (NCBI) 10579
RRIDAB_2200456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95359
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Transforming acidic coiled-coil (TACC) proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. TACC2, also known as anti-zuai-1 (AZU-1), was abundantly expressed in non- and premalignant cells and tissues but was appreciably reduced in breast tumor cell types and in primary tumors, thus TACC2 may act as a tumor suppressor protein and represent a tumor progression marker. Several transcript variants encoding different isoforms have been found for this gene. Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubule and the kinetochore. Centrosomal TACC2 plays a role in organizing centrosomal microtubules and is required for mitotic spindle maintenance.