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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using T7-tag Polyclonal antibody (14441-1-AP)

    WB analysis using 14441-1-AP (same clone as 14441-1-PBS)

    T7-tagged fution protein(20ng) was subjected to SDS PAGE followed by western blot with 14441-1-AP (T7-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14441-1-PBS in a different storage buffer formulation.

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T7-tag Polyclonal antibody, PBS Only

T7-tag Polyclonal Antibody for WB, Indirect ELISA
Cat No. 14441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14441-1-PBS targets T7-tag in WB, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name T7-tag
Calculated molecular weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2878060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The antibody is developed against a synthetic peptide MASMTGGQQMG, the T7 tag, which is present in many vectors.