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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using T7 RNA polymerase Recombinant antibody (84959-4-RR)

    WB analysis of Recombinant protein using 84959-4-RR

    Recombinant protein were subjected to SDS PAGE followed by western blot with 84959-4-RR (T7 RNA polymerase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84959-4-RR

    Affinity and Kinetic Characterization of 84959-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84959-4-RR against T7 RNA polymerase were performed. The affinity constant is 1.84 nM.

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T7 RNA polymerase Recombinant antibody

T7 RNA polymerase Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84959-4-RR
Clone No.242544H3

Host / Isotype

Rabbit / IgG

Reactivity

bacteriophage t7

Applications

WB, ELISA

Synonyms

T7 polymerase, 242544H3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84959-4-RR targets T7 RNA polymerase in WB, ELISA applications and shows reactivity with bacteriophage t7 samples.

Tested Reactivity bacteriophage t7
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen T7 RNA polymerase fusion protein Ag32001 相同性解析による交差性が予測される生物種
Full Name T7 RNA polymerase
Calculated molecular weight99 kDa
GenBank accession numberNP_041960
Gene Symbol T7 RNA polymerase
Gene ID (NCBI) 1261050
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP00573
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for T7 RNA polymerase antibody 84959-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols