Validation Data Gallery
Tested Applications
| Positive WB detected in | MDCK cells, Raji cells, NIH/3T3 cells, mouse spleen tissue, mouse thymus, rat spleen tissue |
| Positive IP detected in | Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87915-1-RR targets Syntaxin 7 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2975 Product name: Recombinant human STX7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC011975 Sequence: MSYTPGVGGDPAQLAQRISSNIQKITQCSVEIQRTLNQLGTPQDSPELRQQLQQKQQYTNQLAKETDKYIKEFGSLPTTPSEQRQRKIQKDRLVAEFTTSLTNFQKVQRQAAEREKEFVARVRASSRVSGSFPEDSSKERNLVSWESQTQPQVQVQDEEITEDDLRLIHERESSIRQLEADIMDINEIFKDLGMMIHEQGDVIDSIEANVENAEVHVQQANQQLSRAADYQRKSRKTLCIIILI 相同性解析による交差性が予測される生物種 |
| Full Name | syntaxin 7 |
| Calculated molecular weight | 261 aa, 30 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC011975 |
| Gene Symbol | Syntaxin 7 |
| Gene ID (NCBI) | 8417 |
| RRID | AB_3745823 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15400 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin 7(STX7) is a SNARE protein localized to late endosomes and lysosomes, mediating vesicle fusion and intracellular trafficking. It plays essential roles in autophagy, pathogen degradation, and cholesterol transport through interactions with VAMP8 and Vti1b.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 7 antibody 87915-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |