Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, mouse kidney tissue, rat kidney tissue, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86181-1-RR targets Syntaxin 4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6973 Product name: Recombinant human STX4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC002436 Sequence: MRDRTHELRQGDDSSDEEDKERVALVVHPGTARLGSPDEEFFHKVRTIRQTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHDIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKTALENQKKARKK 相同性解析による交差性が予測される生物種 |
| Full Name | syntaxin 4 |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 35-37 kDa |
| GenBank accession number | BC002436 |
| Gene Symbol | STX4 |
| Gene ID (NCBI) | 6810 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12846 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin 4 (STX4) is a critical SNARE protein primarily localized to the plasma membrane (PM) in mammalian cells. It plays a fundamental role in vesicle trafficking and fusion, especially in processes requiring rapid, regulated exocytosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 4 antibody 86181-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |