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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Syntabulin Recombinant monoclonal antibody (88360-1-RR)

    WB analysis of HeLa using 88360-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 88360-1-RR (Syntabulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Syntabulin Recombinant monoclonal antibody (88360-1-RR)

    WB analysis of MCF-7 using 88360-1-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 88360-1-RR (Syntabulin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Syntabulin Recombinant monoclonal antibody

Syntabulin Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 88360-1-RR
Clone No.260730D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Golgi-localized syntaphilin-related protein, GOLSYN, KIAA1472, SYBU, Syntaxin-1-binding protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88360-1-RR targets Syntabulin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9344

Product name: Recombinant human GOLSYN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 128-485 aa of BC012082

Sequence: SGRYMSCGENHGVRPPNPEQYLTPLQQKEVTVRHLKTKLKESERRLHERESEIVELKSQLARMREDWIEEECHRVEAQLALKEARKEIKQLKQVIETMRSSLADKDKGIQKYFVDINIQNKKLESLLQSMEMAHSGSLRDELCLDFPCDSPEKSLTLNPPLDTMADGLSLEEQVTGEGADRELLVGDSIANSTDLFDEIVTATTTESGDLELVHSTPGANVLELLPIVMGQEEGSVVVERAVQTDVVPYSPAISELIQSVLQKLQDPCPSSLASPDESEPDSMESFPESLSALVVDLTPRNPNSAILLSPVETPYANVDAEVHANRLMRELDFAACVEERLDGVIPLARGGVVRQYWS

相同性解析による交差性が予測される生物種
Full Name Golgi-localized protein
Calculated molecular weight 662 aa, 72 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC012082
Gene Symbol GOLSYN
Gene ID (NCBI) 55638
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9NX95
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntabulin, also known as GOLSYN or syntaxin-1-binding protein, is a microtubule-associated aptamer protein crucial for intracellular transport. It functions as a linker that attaches syntaxin-1-containing vesicles to the kinesin-1 motor (KIF5B), enabling their anterograde axonal transport. This process is significant for vesicle exocytosis, synapse formation, and neuronal development.

Protocols

Product Specific Protocols
WB protocol for Syntabulin antibody 88360-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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