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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    WB analysis using 27749-1-AP (same clone as 27749-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27749-1-AP (Syndetin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IP experiment of mouse brain using 27749-1-AP (same clone as 27749-1-PBS)

    IP result of anti-Syndetin/CCDC132 (IP:27749-1-AP, 4ug; Detection:27749-1-AP 1:300) with mouse brain tissue lysate 4000 ug. This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of human rectal cancer using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of mouse brain using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of mouse skeletal muscle using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of human rectal cancer using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of mouse brain using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Syndetin/CCDC132 Polyclonal antibody (27749-1-AP)

    IHC staining of human placenta using 27749-1-AP (same clone as 27749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27749-1-AP (Syndetin/CCDC132 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27749-1-PBS in a different storage buffer formulation.

Syndetin/CCDC132 Polyclonal antibody, PBS Only

Syndetin/CCDC132 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 27749-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CCDC132, Syndetin, Coiled-coil domain-containing protein 132, VPS50

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27749-1-PBS targets Syndetin/CCDC132 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26944

Product name: Recombinant human CCDC132 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 337-593 aa of BC152424

Sequence: EWHEKHDNEDTASASEGSNMIGTEETNFDRGYIKKKLEHGLTRIWQDVQLKVKTYLLGTDLSIFKYDDFIFVLDIISRLMQVGEEFCGSKSEVLQESIRKQSVNYFKNYHRTRLDELRMFLENETWELCPVKSNFSILQLHEFKFMEQSRSPSVSPSKQPVSTSSKTVTLFEQYCSGGNPFEIQANHKDEETEDVLASNGYESDEQEKSAYQEYDSDSDVPEELKRDYVDEQTGDGPVKSVSRETLKSRKKSDYSLN

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 132
Calculated molecular weight 964 aa, 111 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC152424
Gene Symbol Syndetin/CCDC132
Gene ID (NCBI) 55610
RRIDAB_2880961
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JG6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Syndetin, also known as CCDC132 or VPS50, is a critical component of the endosome-associated recycling protein (EARP) complex, which also includes ANG2/VPS51, VPS52, and VPS53 in a 1:1:1:1 ratio. The deduced 964-amino acid protein contains alpha-helical N- and C-terminal regions connected by a less structured middle region. CCDC132 is elevated in gastric cancer, where knockdown attenuates proliferation and tumorigenesis by facilitating DNA damage signaling, and its expression correlates with nodal metastasis and poor prognosis.

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