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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of BxPC-3 cells using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    WB analysis of BxPC-3 using 84452-1-RR (same clone as 84452-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    WB analysis using 84452-1-RR (same clone as 84452-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    IHC staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    IHC staining of rat brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    IHC staining of rat brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Synaptotagmin-5 Recombinant antibody (84452-1-RR)

    IF Staining of mouse brain using 84452-1-RR (same clone as 84452-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84452-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84452-1-RR

    Affinity and Kinetic Characterization of 84452-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

Synaptotagmin-5 Recombinant monoclonal antibody, PBS Only

Synaptotagmin-5 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84452-1-PBS
Clone No.241452E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

SytV, SYT5, Synaptotagmin V, 241452E3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84452-1-PBS targets Synaptotagmin-5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag12677

Product name: Recombinant human SYT5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 51-386 aa of BC046157

Sequence: YRKSCRRRTGKKSQAQAQVHLQEVKGLGQSYIDKVQPEVEELEPAPSGPGQQVADKHELGQLQYSLDYDFQSGQLLVGILQAMGLAALDLGGSSDPYVRVYLLPDKRRRYETKVHRQTLNPHFGETFAFKVPYVELGGRVLVMAVYDFDRFSRNDAIGEVRVPMSSVDLGRPVQAWRELQAAPREEQEKLGDICFSLRYVPTAGKLTVIVLEAKNLKKMDVGGLSDPYVKVHLLQGGKKVRKKKTTIKKNTLNPYYNEAFSFEVPCDQVQKVQVELTVLDYDKLGKNEAIGRVAVGAAAGGAGLRHWADMLANPRRPIAQWHSLRPPDRVRLLPAP

相同性解析による交差性が予測される生物種
Full Name synaptotagmin V
Calculated molecular weight 43 kDa
Observed molecular weight42 kDa
GenBank accession numberBC046157
Gene Symbol Synaptotagmin-5
Gene ID (NCBI) 6861
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO00445
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).

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