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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-14 Polyclonal antibody (20561-1-AP)

    WB analysis using 20561-1-AP (same clone as 20561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Polyclonal antibody (20561-1-AP)

    IHC staining of mouse cerebellum using 20561-1-AP (same clone as 20561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-14 Polyclonal antibody (20561-1-AP)

    IHC staining of mouse cerebellum using 20561-1-AP (same clone as 20561-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20561-1-AP (Synaptotagmin-14 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20561-1-PBS in a different storage buffer formulation.

Synaptotagmin-14 Polyclonal antibody, PBS Only

Synaptotagmin-14 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20561-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Synaptotagmin XIV, SYT14, SytXIV

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20561-1-PBS targets Synaptotagmin-14 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name synaptotagmin XIV
Calculated molecular weight 62 kDa
Observed molecular weight62-65 kDa
GenBank accession numberNM_153262
Gene Symbol Synaptotagmin-14
Gene ID (NCBI) 255928
RRIDAB_3085607
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NB59
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-14 (SYT14) is highly expressed in brain tissues. A homozygous mutation of SYT14 is involved in the pathogenesis of spinocerebellar ataxia (SCA) (PMID: 21835308).

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