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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis using 55015-1-AP (same clone as 55015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of mouse brain using 55015-1-AP (same clone as 55015-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55015-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Synaptotagmin-12 Polyclonal antibody (55015-1-AP)

    WB analysis of SH-SY5Y using 55015-1-AP (same clone as 55015-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 55015-1-AP (Synaptotagmin-12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55015-1-PBS in a different storage buffer formulation.

Synaptotagmin-12 Polyclonal antibody, PBS Only

Synaptotagmin-12 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 55015-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Synaptotagmin XII, SYT12, SytXII

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55015-1-PBS targets Synaptotagmin-12 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name synaptotagmin XII
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_177963
Gene Symbol Synaptotagmin-12
Gene ID (NCBI) 91683
RRIDAB_10859791
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IV01
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYT12 belongs to the synaptotagmin family. It may be involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain or may serve as Ca2+ sensors in the process of vesicular trafficking and exocytosis. The antibody is specific to STY12.

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