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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis using 68043-1-Ig (same clone as 68043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis of rat cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    WB analysis of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse brain using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Synaptotagmin-1 Monoclonal antibody (68043-1-Ig)

    IHC staining of mouse cerebellum using 68043-1-Ig (same clone as 68043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68043-1-Ig (Synaptotagmin-1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68043-1-PBS in a different storage buffer formulation.

Synaptotagmin-1 Monoclonal antibody, PBS Only

Synaptotagmin-1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68043-1-PBS
Clone No.1H1H2

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit, Chicken

Applications

WB, IHC, Indirect ELISA

Synonyms

DKFZp781D2042, P65, SVP65, Synaptotagmin 1, synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68043-1-PBS targets Synaptotagmin-1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit, Chicken samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit, Chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag6071

Product name: Recombinant human SYT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 97-422 aa of BC058917

Sequence: GGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEEEKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQFTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFSLRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPFEQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAVKK

相同性解析による交差性が予測される生物種
Full Name synaptotagmin I
Calculated molecular weight 48 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC058917
Gene Symbol Synaptotagmin-1
Gene ID (NCBI) 6857
RRIDAB_2918785
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP21579
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114).

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