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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Surfactant protein D Polyclonal antibody (33001-1-AP)

    WB analysis using 33001-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33001-1-AP (Surfactant protein D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant protein D Polyclonal antibody (33001-1-AP)

    IHC staining of mouse lung using 33001-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 33001-1-AP (Surfactant protein D antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat lung tissue using Surfactant protein D Polyclonal antibody (33001-1-AP)

    IHC staining of rat lung using 33001-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 33001-1-AP (Surfactant protein D antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Surfactant protein D Polyclonal antibody

Surfactant protein D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 33001-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Lung surfactant protein D, PSP-D, Pulmonary surfactant-associated protein D, Sftp4, Sftpd

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse lung tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

33001-1-AP targets Surfactant protein D in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg2924

Product name: Recombinant Mouse Surfactant protein D protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 20-374 aa of NM_009160.2

Sequence: AEMKSLSQRSVPNTCTLVMCSPTENGLPGRDGRDGREGPRGEKGDPGLPGPMGLSGLQGPTGPVGPKGENGSAGEPGPKGERGLSGPPGLPGIPGPAGKEGPSGKQGNIGPQGKPGPKGEAGPKGEVGAPGMQGSTGAKGSTGPKGERGAPGVQGAPGNAGAAGPAGPAGPQGAPGSRGPPGLKGDRGVPGDRGIKGESGLPDSAALRQQMEALKGKLQRLEVAFSHYQKAALFPDGRSVGDKIFRTADSEKPFEDAQEMCKQAGGQLASPRSATENAAIQQLITAHNKAAFLSMTDVGTEGKFTYPTGEPLVYSNWAPGEPNNNGGAENCVEIFTNGQWNDKACGEQRLVICEF

相同性解析による交差性が予測される生物種
Full Name surfactant associated protein D
Calculated molecular weight38 kDa
Observed molecular weight40 kDa
GenBank accession numberNM_009160.2
Gene Symbol Sftpd
Gene ID (NCBI) 20390
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDP50404
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Surfactant protein D (SP-D) is a pattern recognition molecule of the collectin family of C-type lectins, which consist of four structural domains: a cysteine-rich N-terminal domain, a collagen-like region, an alpha-helical coiled-coil neck domain and a C-terminal lectin or carbohydrate-recognition domain (PMID: 16213021; 15030473). SP-D is primarily expressed and secreted by type II alveolar cells (PMID: 16684127). It plays a central role in the pulmonary host defence and the modulation of allergic responses.

Protocols

Product Specific Protocols
WB protocol for Surfactant protein D antibody 33001-1-APDownload protocol
IHC protocol for Surfactant protein D antibody 33001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols