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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IHC staining of mouse lung using 86322-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86322-1-RR (Surfactant Protein A antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IHC staining of mouse lung using 86322-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 86322-1-RR (Surfactant Protein A antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IF Staining of mouse lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IF Staining of rat lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IF Staining of human lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung tissue using Surfactant Protein A Recombinant monoclonal antibo (86322-1-RR)

    IF Staining of human lung using 86322-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung tissue using Surfactant Protein A antibody (86322-1-RR, Clone: 250912G10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Surfactant Protein A Recombinant monoclonal antibody

Surfactant Protein A Uni-rAb® Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 86322-1-RR
Clone No.250912G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

SFTPA1, SFTPA2, 35 kDa pulmonary surfactant-associated protein, COLEC4, Collectin 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung tissue, rat lung tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86322-1-RR targets Surfactant Protein A in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2417

Product name: Recombinant human SFTPA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 132-248 aa of BC026229

Sequence: MTVGEKVFSSNGQSITFDAIQEACARAGGRIAVPRNPEENEAIASFVKKYNTYAYVGLTEGPSPGDFRYSDGTPVNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRNCLYSRLTICEF

相同性解析による交差性が予測される生物種
Full Name surfactant protein A1
Calculated molecular weight 248 aa, 26 kDa
GenBank accession numberBC026229
Gene Symbol Surfactant Protein A
Gene ID (NCBI) 653509
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8IWL2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Surfactant protein A (SP-A), is the major protein component of pulmonary surfactant involved in surfactant-related function or structure and in the regulation of inflammatory processes and innate host defens. In humans and primates, SP-A1 (SFTPA1) and SP-A2 (SFTPA2) genes encode SP-A, and these two SP-A genes have high homology (PMID: 34484180, PMID: 19392648). This antibody can recognize both SP-A1 (SFTPA1) and SP-A2 (SFTPA2).

Protocols

Product Specific Protocols
IF protocol for Surfactant Protein A antibody 86322-1-RRDownload protocol
IHC protocol for Surfactant Protein A antibody 86322-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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