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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human placenta using 67894-1-Ig (same clone as 67894-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human testis using 67894-1-Ig (same clone as 67894-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of HeLa using 67894-1-Ig (same clone as 67894-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67894-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using Stomatin Monoclonal antibody (67894-1-Ig)

    WB analysis of human peripheral blood platelets using 67894-1-Ig (same clone as 67894-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67894-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Stomatin Monoclonal antibody (67894-1-Ig)

    IHC staining of human lung cancer using 67894-1-Ig (same clone as 67894-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67894-1-Ig (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67894-1-PBS in a different storage buffer formulation.

Stomatin Monoclonal antibody, PBS Only

Stomatin Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67894-1-PBS
Clone No.1A4D6

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67894-1-PBS targets Stomatin in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag2676

Product name: Recombinant human STOM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-288 aa of BC010703

Sequence: MAEKRHTRDSEAQRLPDSFKDSPSKGLGPCGWILVAFSFLFTVITFPISIWMCIKIIKEYERAIIFRLGRILQGGAKGPGLFFILPCTDSFIKVDMRTISFDIPPQEILTKDSVTISVDGVVYYRVQNATLAVANITNADSATRLLAQTTLRNVLGTKNLSQILSDREEIAHNMQSTLDDATDAWGIKVERVEIKDVKLPVQLQRAMAAEAEASREARAKVIAAEGEMNASRALKEASMVITEYPAALQLRYLQTLTTIAAEKNSTIVFPLPIDMLQGIIGAKHSHLG

相同性解析による交差性が予測される生物種
Full Name stomatin
Calculated molecular weight 288 aa, 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC010703
Gene Symbol Stomatin
Gene ID (NCBI) 2040
RRIDAB_2918650
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP27105
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)