Validation Data Gallery
![Western blot analysis of N- and C-terminal Spot-tagged GFP added to HEK- 293T cell lysate. Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1, ChromoTek) 1:5,000. Western blot membrane was incubated simultaneously with the primary antibody and Nano-Secondary® (one-step staining).](https://www.ptglab.com/products/pictures/28A5-1.jpg "Western blot analysis of N- and C-terminal Spot-tagged GFP added to HEK- 293T cell lysate. Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1, ChromoTek) 1:5,000. Western blot membrane was incubated simultaneously with the primary antibody and Nano-Secondary® (one-step staining).")
![Western blot analysis of C-terminal Spot-tagged GFP added to HEK-293T cell lysate. Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and anti-mouse secondary antibody HRP 1:1,000.](https://www.ptglab.com/products/pictures/28A5-2.png "Western blot analysis of C-terminal Spot-tagged GFP added to HEK-293T cell lysate. Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and anti-mouse secondary antibody HRP 1:1,000.")
![Western blot analysis of different cell lysates +/- 50 ng C-terminal Spot-tagged GFP: human (HEK-293T), yeast (S.cerevisiae), insect (High Five™), bacteria (E.coli) and plant (A.thaliana). Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1, ChromoTek) 1:5,000. Western blot membrane was incubated simultaneously with the primary antibody and Nano-Secondary® (one-step staining).](https://www.ptglab.com/products/pictures/Result-28a5_20210803100622724.png "Western blot analysis of different cell lysates +/- 50 ng C-terminal Spot-tagged GFP: human (HEK-293T), yeast (S.cerevisiae), insect (High Five™), bacteria (E.coli) and plant (A.thaliana). Detection with Spot-tag® antibody [28A5] (28a5, ChromoTek) 1:5,000 and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] (sms1AF488-1, ChromoTek) 1:5,000. Western blot membrane was incubated simultaneously with the primary antibody and Nano-Secondary® (one-step staining).")
![ELISA detection of N- and C-terminal Spot-tagged GFP with Spot-tag® antibody [28A5] (28a5, ChromoTek). N- and C-terminal Spot-tagged GFP was coated on a microtiter plate, blocked and detected with 1 nM Spot-tag® antibody [28A5] (28a5, ChromoTek) and anti-mouse IgG antibody AP.](https://www.ptglab.com/products/pictures/Result-28a5_20210803100844572.png "ELISA detection of N- and C-terminal Spot-tagged GFP with Spot-tag® antibody [28A5] (28a5, ChromoTek). N- and C-terminal Spot-tagged GFP was coated on a microtiter plate, blocked and detected with 1 nM Spot-tag® antibody [28A5] (28a5, ChromoTek) and anti-mouse IgG antibody AP.")
Product Information
Mouse monoclonal antibody to Spot-tag®
| Description | Spot-tag® mouse monoclonal [28A5] for WB, ELISA |
| Applications | WB, ELISA |
| Host/IsoType | Mouse/IgG1 |
| Specificity/Target | Spot-Tag® (PDRVRAVSHWSS) at the N-terminus, C-terminus, or internal site of the fusion protein |
| Conjugate | Unconjugated |
| Recommended Dilution | WB: 1:1,000 - 1:5,000 ELISA: 1:5,000 - 1:10,000 |
| Purification Method | Protein A purification |
| Type | Primary Antibody |
| Class | Monoclonal |
| Format | Monoclonal mouse antibody |
| Immunogen | Spot-tagged fusion protein |
| Clone No. | 28A5 |
| Storage Buffer | PBS, 50% glycerol |
| Storage Condition | Shipped on ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Form | Liquid |
| Size | 20 μL; 100 μL |
| RRID | AB_2892245 |
Publications
| Application | Title |
|---|---|
iScience Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors | |
Methods Mol Biol Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP) | |
Mol Cell Anti-apoptotic MCL-1 promotes long-chain fatty acid oxidation through interaction with ACSL1 | |
Open Biol The cytosolic form of dual localized BolA family protein Bol3 is important for adaptation to iron starvation in Aspergillus fumigatus |