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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Sortilin Monoclonal antibody (68007-1-Ig)

    WB analysis using 68007-1-Ig (same clone as 68007-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Sortilin Monoclonal antibody (68007-1-Ig)

    WB analysis using 68007-1-Ig (same clone as 68007-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of human lung cancer using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IHC staining of human lung cancer using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Sortilin Monoclonal antibody (68007-1-Ig)

    IF Staining of mouse brain using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Sortilin Monoclonal antibody (68007-1-Ig)

    IF Staining of HeLa using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Sortilin Monoclonal antibody (68007-1-Ig)

    IF Staining of SH-SY5Y using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using Sortilin Monoclonal antibody (68007-1-Ig)

    IF Staining of SH-SY5Y using 68007-1-Ig (same clone as 68007-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68007-1-PBS in a different storage buffer formulation.

Sortilin Monoclonal antibody, PBS Only

Sortilin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 68007-1-PBS
Clone No.2B11B9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68007-1-PBS targets Sortilin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28043

Product name: Recombinant human SORT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 481-831 aa of BC023542

Sequence: EPNAVGIVIAHGSVGDAISVMVPDVYISDDGGYSWTKMLEGPHYYTILDSGGIIVAIEHSSRPINVIKFSTDEGQCWQTYTFTRDPIYFTGLASEPGARSMNISIWGFTESFLTSQWVSYTIDFKDILERNCEEKDYTIWLAHSTDPEDYEDGCILGYKEQFLRLRKSSVCQNGRDYVVTKQPSICLCSLEDFLCDFGYYRPENDSKCVEQPELKGHDLEFCLYGREEHLTTNGYRKIPGDKCQGGVNPVREVKDLKKKCTSNFLSPEKQNSKSNSVPIILAIVGLMLVTVVAGVLIVKKYVCGGRFLVHRYSVLQQHAEANGVDGVDALDTASHTNKSGYHDDSDEDLLE

相同性解析による交差性が予測される生物種
Full Name sortilin 1
Calculated molecular weight 831 aa, 92 kDa
Observed molecular weight95-100 kDa
GenBank accession numberBC023542
Gene Symbol Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2918754
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ99523
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

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