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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Snf1lk Recombinant antibody (82851-3-RR)

    WB analysis using 82851-3-RR (same clone as 82851-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82851-3-RR (Snf1lk antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82851-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Snf1lk Recombinant antibody (82851-3-RR)

    WB analysis of HeLa using 82851-3-RR (same clone as 82851-3-PBS)

    WB result of Snf1lk antibody (82851-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-Snf1lk transfected HeLa cells. This data was developed using the same antibody clone with 82851-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82851-3-RR

    Affinity and Kinetic Characterization of 82851-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82851-3-RR against Mouse Snf1lk were performed. The affinity constant is 0.686 nM.

  • Featured Product
  • KD/KO Validated

Mouse Snf1lk Recombinant monoclonal antibody, PBS Only

Snf1lk Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 82851-3-PBS
Clone No.3D2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Sik1, EC:2.7.11.1, HRT-20, Msk, Myocardial SNF1-like kinase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82851-3-PBS targets Snf1lk in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag33435

Product name: Recombinant mouse Snf1lk protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 423-769 aa of NM_010831

Sequence: LDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPCIIVSSSATASPSEGTSSDSCLPFSASEGPAGLGSGLATPGLLGTSSPVRLASPFLGSQSATPVLQTQAGLGTAVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSVRTPRGGMSTFHTPAPSSGLQGCTTSNREGRSLLEEVLHQQRLLQLQHHSSTAAASSGCQQGPQLSPVPYVLAPCDSLLVSGIPLLPTPLLQAGMSPVASAAHLLDTHLHISAGPVALPTGPLPQCLTRLSPGCDPAGLPQGDCEMEDLTS

相同性解析による交差性が予測される生物種
Full Name salt inducible kinase 1
Calculated molecular weight 85 kDa
Observed molecular weight85 kDa
GenBank accession numberNM_010831
Gene Symbol Sik1
Gene ID (NCBI) 17691
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ60670
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.