Product Information
SignalBright Pro is a luminol-based, chemiluminescent substrate for HRP (horseradish peroxidase) which provides a much higher sensitivity over traditional ECL reagents. SignalBright Pro provides mid to high- femtogram detection levels in Western Blot and ELISA analysis, with long lasting signal up to 24 hours.
Key Characteristics:
· Sensitivity: Mid to high femtogram
· Signal duration: >12 hours
· Compatibility: suitable for PVDF and nitrocellulose membranes
· Comparable sensitivity to SuperSignal™ West Dura.
Instructions for Use
SignalBright Pro Chemiluminescent Detection is prepared by combining a 1:1 ratio of the Chemiluminescent Substrate Solution and Stable Peroxide Solution to create a working solution. Allow the working solution to come to room temperature before applying to the membrane, make sure this is done in darkness. Apply a minimum of 0.1ml of the working solution per membrane cm2. Leave 5 minutes for signal to develop prior to imaging.
· Recommended primary antibody concentration: 0.02-1 ug/ml
· Recommended HRP-conjugated secondary antibody concentration: 4-20 ng/ml
Product Information
| Cat. | Size | Content |
| PK10011 | 20mL | 10mL Chemiluminescent Substrate Solution |
| 10mL Stable Peroxide Solution | ||
| 100mL | 50mL Chemiluminescent Substrate Solution | |
| 50mL Stable Peroxide Solution |
Storage
Store at 4ºC. Expires 1 year after purchase.
Cited in Article as
pk10011, SignalBright Pro Chemiluminescent Substrate, Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Am J Physiol Cell Physiol Insulin potentiates mechanical responses in small dorsal root ganglion neurons by increasing the sensitization of TRPV4 channels |