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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human plasma using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    WB analysis of human plasma using 66084-1-Ig (same clone as 66084-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66084-1-Ig (Serum amyloid P component antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IHC staining of human kidney using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66084-1-Ig(Serum amyloid P component antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver cancer using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of mouse spleen using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver tissue using Serum amyloid P component Monoclonal antibody (66084-1-Ig)

    IF Staining of human liver using 66084-1-Ig (same clone as 66084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Serum amyloid P component antibody (66084-1-Ig, Clone: 1A1D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66084-1-PBS in a different storage buffer formulation.

Serum amyloid P component Monoclonal antibody, PBS Only

Serum amyloid P component Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66084-1-PBS
Clone No.1A1D10

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

APCS, SAP, 1A1D10, 9.5S alpha 1 glycoprotein, 9.5S alpha-1-glycoprotein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66084-1-PBS targets Serum amyloid P component in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag14957

Product name: Recombinant human APCS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 20-223 aa of BC007058

Sequence: HTDLSGKVFVFPRESVTDHVNLITPLEKPLQNFTLCFRAYSDLSRAYSLFSYNTQGRDNELLVYKERVGEYSLYIGRHKVTSKVIEKFPAPVHICVSWESSSGIAEFWINGTPLVKKGLRQGYFVEAQPKIVLGQEQDSYGGKFDRSQSFVGEIGDLYMWDSVLPPENILSAYQGTPLPANILDWQALNYEIRGYVIIKPLVWV

相同性解析による交差性が予測される生物種
Full Name amyloid P component, serum
Calculated molecular weight 223 aa, 25 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
RRIDAB_11182271
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02743
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Serum amyloid P component (SAP), a member of pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.