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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Serpin F1/PEDF Recombinant antibody (84685-3-RR)

    WB analysis of HepG2 using 84685-3-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C2C12 cells using Serpin F1/PEDF Recombinant antibody (84685-3-RR)

    WB analysis of C2C12 using 84685-3-RR

    C2C12 cells were subjected to SDS PAGE followed by western blot with 84685-3-RR (Serpin F1/PEDF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using Serpin F1/PEDF Recombinant antibody (84685-3-RR)

    FC experiment of HEK-293 using 84685-3-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Serpin F1/PEDF Recombinant antibody (84685-3-RR, Clone:242127F9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84685-3-RR

    Affinity and Kinetic Characterization of 84685-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84685-3-RR against Human Serpin F1/PEDF were performed. The affinity constant is 1.08 nM.

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Serpin F1/PEDF Recombinant antibody

Serpin F1/PEDF Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84685-3-RR
Clone No.242127F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

PEDF, PIG35, 242127F9, Cell proliferation-inducing gene 35 protein, EPC 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84685-3-RR targets Serpin F1/PEDF in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
Calculated molecular weight46kDa
Observed molecular weight 46 kDa
GenBank accession numberNM_002615.7
Gene Symbol PEDF
Gene ID (NCBI) 5176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP36955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Protocols

Product Specific Protocols
WB protocol for Serpin F1/PEDF antibody 84685-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols