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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    WB analysis of human plasma using 66052-1-Ig (same clone as 66052-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Serpin C1/Antithrombin III antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    WB analysis of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    WB analysis of human testis using 66052-1-Ig (same clone as 66052-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    WB analysis of human brain using 66052-1-Ig (same clone as 66052-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    WB analysis of rat brain using 66052-1-Ig (same clone as 66052-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66052-1-Ig (Antithrombin III antibody at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IHC staining of human liver cancer using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IHC staining of human liver using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66052-1-Ig (Antithrombin III antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IF Staining of HepG2 using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Serpin C1/Antithrombin III antibody (66052-1-Ig, Clone: 2B2E5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Serpin C1/Antithrombin III Monoclonal antibody (66052-1-Ig)

    IF Staining of L02 using 66052-1-Ig (same clone as 66052-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol ) fixed L02 cells using 66052-1-Ig(Antithrombin III antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66052-1-PBS in a different storage buffer formulation.

Serpin C1/Antithrombin III Monoclonal antibody, PBS Only

Serpin C1/Antithrombin III Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66052-1-PBS
Clone No.2B2E5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Antithrombin III, Serpin C1, SERPINC1, 2B2E5, Antithrombin-III

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66052-1-PBS targets Serpin C1/Antithrombin III in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9880

Product name: Recombinant human SERPINC1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-259 aa of BC022309

Sequence: MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLADSKNDNDNIFLSPLSISTAFAMTKLGACNDTLQQLMEVFKFDTISEKTSDQIHFFFAKLNCRLYRKANKSSKLVSANRLFGDKSLTFNDLYVSDAFHKAFLEVNEEGSEAAASTAVVIAGRSLNPNRVTFKANRPFLVFIREVPLNTIIFMGRVANPCVK

相同性解析による交差性が予測される生物種
Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Calculated molecular weight 464 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC022309
Gene Symbol Serpin C1/Antithrombin III
Gene ID (NCBI) 462
RRIDAB_11042589
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01008
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SERPINC1, also named Antithrombin III or Antithrombin, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.

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