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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis using 10222-1-AP (same clone as 10222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10222-1-AP (Sam68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis of NIH/3T3 using 10222-1-AP (same clone as 10222-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis of mouse brain using 10222-1-AP (same clone as 10222-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using Sam68 Polyclonal antibody (10222-1-AP)

    WB analysis of HEK-293 using 10222-1-AP (same clone as 10222-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10222-1-AP (KHDRBS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using Sam68 Polyclonal antibody (10222-1-AP)

    IP experiment of HeLa using 10222-1-AP (same clone as 10222-1-PBS)

    IP result of anti-Sam68 (IP:10222-1-AP, 4ug; Detection:10222-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Sam68 Polyclonal antibody (10222-1-AP)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Sam68 Polyclonal antibody (10222-1-AP)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Sam68 Polyclonal antibody (10222-1-AP)

    IHC staining of mouse colon using 10222-1-AP (same clone as 10222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10222-1-AP (Sam68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Sam68 Polyclonal antibody (10222-1-AP)

    IF Staining of HepG2 using 10222-1-AP (same clone as 10222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Sam68 antibody (10222-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 10222-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Sam68 Polyclonal antibody, PBS Only

Sam68 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10222-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

KHDRBS1, GAP-associated tyrosine phosphoprotein p62, KH domain-containing, RNA-binding, signal transduction-associated protein 1, KHDRBS 1, p21 Ras GTPase-activating protein-associated p62

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10222-1-PBS targets Sam68 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0235

Product name: Recombinant human Sam68 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 201-442 aa of BC000717

Sequence: GSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLNGVPEPSRGRGVPVRGRGAAPPPPPVPRGRGVGPPRGALVRGTPVRGAITRGATVTRGVPPPPTVRGAPAPRARTAGIQRIPLPPPPAPETYEEYGYDDTYAEQSYEGYEGYYSQSQGDSEYYDYGHGEVQDSYEAYGQDDWNGTRPSLKAPPARPVKGAYREHPYGR

相同性解析による交差性が予測される生物種
Full Name KH domain containing, RNA binding, signal transduction associated 1
Calculated molecular weight 48 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC000717
Gene Symbol Sam68
Gene ID (NCBI) 10657
RRIDAB_2130292
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ07666
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

KHDRBS1, also named as SAM68, p62and p68, belongs to the KHDRBS family. It is recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and INS receptors. Once phosphorylated, KHDRBS1 functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. It represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. KHDRBS1 also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. KHDRBS1 has three isoforms with calculated MW of 44-48kd. For isoform with a calculated MW of 48.2 kD, it migrated as a 68kD protein on SDS-PAGE gels[PMID: 10564820]. This ia a rabbit polyclonal antibody raised against residues near the N terminus of human Sam68.

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