Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, A431 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20472-1-AP targets Synaptotagmin-4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14318 Product name: Recombinant human SYT4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-187 aa of BC036538 Sequence: MAPITTSREEFDEIPTVVGIFSAFGLVFTVSLFAWICCQRKSSKSNKTPPYKFVHVLKGVDIYPENLNSKKKFGADDKNEVKNKPAVPKNSLHLDLEKRDLNGNFPKTNLKPGSPSDLENATPKLFLEGEKESVSPESLKSSTSLTSEEKQEKLGTLFFSLEYNFERKAFVVNIKEARGLPAMDEQS 相同性解析による交差性が予測される生物種 |
| Full Name | synaptotagmin IV |
| Calculated molecular weight | 425 aa, 48 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC036538 |
| Gene Symbol | Synaptotagmin-4 |
| Gene ID (NCBI) | 6860 |
| RRID | AB_10695633 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2B2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-4 is an atypical synaptic vesicle protein that is expressed in the dendrites of neurons. It mediates retrograde signals at the synapse and the release of BDNF, and regulates synaptic plasticity, motor learning, and memory.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptotagmin-4 antibody 20472-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |