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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Synaptotagmin-4 Polyclonal antibody

Synaptotagmin-4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12642-1-AP

Synonyms

HsT1192, KIAA1342, Synaptotagmin 4, synaptotagmin IV, Synaptotagmin-4, SYT4, SytIV

Validation Data Gallery

Applications:
  • WB analysis of human brain using 12642-1-AP

    WB analysis of human brain using 12642-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 12642-1-AP

    WB analysis of human brain using 12642-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 12642-1-AP

    IHC staining of human gliomas using 12642-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 12642-1-AP (Synaptotagmin-4 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12642-1-AP targets Synaptotagmin-4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptotagmin-4 fusion protein Ag3338 相同性解析による交差性が予測される生物種
Full Name synaptotagmin IV
Calculated molecular weight 425 aa, 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC036538
Gene symbol SYT4
Gene ID (NCBI) 6860
RRIDAB_2302982
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Synaptotagmin-4 antibody 12642-1-APDownload protocol
IHC protocol for Synaptotagmin-4 antibody 12642-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Histochem Cell Biol

Significance of achaete-scute complex homologue 1 (ASCL1) in pulmonary neuroendocrine carcinomas; RNA sequence analyses using small cell lung cancer cells and Ascl1-induced pulmonary neuroendocrine carcinoma cells.

Authors - Shinji Kudoh
View Article
humanWB

J Biol Chem

Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent manners

Authors - Yidan Xu
View Article