Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
18115-1-AP targets Synaptotagmin-13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synaptotagmin-13 fusion protein Ag12599 相同性解析による交差性が予測される生物種 |
| Full Name | synaptotagmin XIII |
| Calculated molecular weight | 426 aa, 47 kDa |
| Observed molecular weight | 47 kDa, 66 kDa |
| GenBank accession number | BC093830 |
| Gene Symbol | Synaptotagmin-13 |
| Gene ID (NCBI) | 57586 |
| RRID | AB_2199453 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L8C5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Synaptotagmin-13 is a member of the synaptotagmin family of proteins which are known as membrane trafficking proteins. Synaptotagmin-13 is composed of the canonical domains of synaptotagmins that include an N-terminal transmembrane region, two C-terminal cytoplasmic C2-domains, and a connecting sequence between the transmembrane region and the C2-domains (PMID: 11211934). Synaptotagmin-13 is highly expressed in brain and may be involved in transport vesicle docking to the plasma membrane.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Synaptotagmin-13 antibody 18115-1-AP | Download protocol |
| IHC protocol for Synaptotagmin-13 antibody 18115-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Key role of SMN/SYNCRIP and RNA-Motif 7 in spinal muscular atrophy: RNA-Seq and motif analysis of human motor neurons. |