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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYNPO2L Recombinant antibody (83532-3-RR)

    IF Staining of HepG2 using 83532-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (83532-3-RR, Clone: 240523F12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Caco-2 cells using SYNPO2L Recombinant antibody (83532-3-RR)

    FC experiment of Caco-2 using 83532-3-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug SYNPO2L Recombinant antibody (83532-3-RR, Clone:240523F12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83532-3-RR

    Affinity and Kinetic Characterization of 83532-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83532-3-RR against Human SYNPO2L were performed. The affinity constant is 80.5 pM.

SYNPO2L Recombinant monoclonal antibody

SYNPO2L Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83532-3-RR
Clone No.240523F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Synaptopodin 2-like protein, Synaptopodin 2 like protein, 240523F12

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83532-3-RR targets SYNPO2L in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34027

Product name: Recombinant human SYNPO2L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 35-389 aa of BC142635

Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR

相同性解析による交差性が予測される生物種
Full Name synaptopodin 2-like
Calculated molecular weight 977 aa, 102 kDa
GenBank accession numberBC142635
Gene Symbol SYNPO2L
Gene ID (NCBI) 79933
RRIDAB_3671157
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H987
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).

Protocols

Product Specific Protocols
IF protocol for SYNPO2L antibody 83532-3-RRDownload protocol
FC protocol for SYNPO2L antibody 83532-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols