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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis using 21480-1-AP (same clone as 21480-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis of PC-3 using 21480-1-AP (same clone as 21480-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    WB analysis of mouse testis using 21480-1-AP (same clone as 21480-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    IHC staining of mouse heart using 21480-1-AP (same clone as 21480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SYNPO2L Polyclonal antibody (21480-1-AP)

    IHC staining of mouse heart using 21480-1-AP (same clone as 21480-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SYNPO2L Polyclonal antibody (21480-1-AP)

    IF Staining of HepG2 using 21480-1-AP (same clone as 21480-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (21480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21480-1-PBS in a different storage buffer formulation.

SYNPO2L Polyclonal antibody, PBS Only

SYNPO2L Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21480-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21480-1-PBS targets SYNPO2L in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15683

Product name: Recombinant human SYNPO2L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 35-389 aa of BC142635

Sequence: LQRAESLQEKSIKEAKTKCRTIASLLTAAPNPHSKGVLMFKKRRQRAKKYTLVSFGAAAGTGAEEEDGVPPTSESELDEEAFSDARSLTNQSDWDSPYLDMELARAGSRASEGQGSGLGGQLSEVSGRGVQLFEQQRQRADSSTQELARVEPAAMLNGEGLQSPPRAQSAPPEAAVLPPSPLPAPVASPRPFQPGGGAPTPAPSIFNRSARPFTPGLQGQRPTTTSVIFRPLAPKRANDSLGGLSPAPPPFLSSQGPTPLPSFTSGVPSHAPVSGSPSTPRSSGPVTATSSLYIPAPSRPVTPGGAPEPPAPPSAAAMTSTASIFLSAPLRPSARPEAPAPGPGAPEPPSAREQR

相同性解析による交差性が予測される生物種
Full Name synaptopodin 2-like
Calculated molecular weight 977 aa, 102 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC142635
Gene Symbol SYNPO2L
Gene ID (NCBI) 79933
RRIDAB_10860260
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H987
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).