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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    WB analysis of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    WB analysis of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    WB analysis of pig brain using 67339-1-Ig (same clone as 67339-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67339-1-Ig (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    IHC staining of rat brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    IHC staining of human kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67339-1-Ig (SYNPO antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    IF Staining of mouse brain using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using SYNPO Monoclonal antibody (67339-1-Ig)

    IF Staining of mouse kidney using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SYNPO Monoclonal antibody (67339-1-Ig)

    IF Staining of HEK-293 using 67339-1-Ig (same clone as 67339-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SYNPO antibody (67339-1-Ig, Clone: 1E1B1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67339-1-PBS in a different storage buffer formulation.

SYNPO Monoclonal antibody, PBS Only

SYNPO Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 67339-1-PBS
Clone No.1E1B1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

Synaptopodin, 1E1B1, KIAA1029, snaptopodin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67339-1-PBS targets SYNPO in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15089

Product name: Recombinant human SYNPO protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 336-685 aa of BC146665

Sequence: SPPSYSVLYPSSDPKSSHLKGQAVPASKTGILEESMARRGSRKSMFTFVEKPKVTPNPDLLDLVQTADEKRRQRDQGEVGVEEEPFALGAEASNFQQEPAPRDRASPAAAEEVVPEWASCLKSPRIQAKPKPKPNQNLSEASGKGAELYARRQSRMEKYVIESSSHTPELARCPSPTMSLPSSWKYPTNAPGAFRVASRSPARTPPASLYHGYLPENGVLRPEPTKQPPYQLRPSLFVLSPIKEPAKVSPRAASPAKPSSLDLVPNLPKGALPPSPALPRPSRSSPGLYTSPGQDSLQPTAVSPPYGGDISPVSPSRAWSPRAKQAPRPSFSTRNAGIEAQVWKPSFCFK

相同性解析による交差性が予測される生物種
Full Name synaptopodin
Calculated molecular weight 929 aa, 99 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2882597
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ8N3V7
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

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