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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNM Polyclonal antibody (20735-1-AP)

    WB analysis using 20735-1-AP (same clone as 20735-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. SYNM gene methylation in T-47D cells causes gene silencing. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using SYNM Polyclonal antibody (20735-1-AP)

    WB analysis of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20735-1-AP (SYNM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human skeletal muscle using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human heart using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human heart using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human bladder using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using SYNM Polyclonal antibody (20735-1-AP)

    IHC staining of human bladder using 20735-1-AP (same clone as 20735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder using 20735-1-AP (SYNM antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SYNM Polyclonal antibody (20735-1-AP)

    IF Staining of HeLa using 20735-1-AP (same clone as 20735-1-PBS)

    Immunofluorescent analysis of HeLa cells using 20735-1-AP (SYNM antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 20735-1-PBS in a different storage buffer formulation.

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SYNM Polyclonal antibody, PBS Only

SYNM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20735-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20735-1-PBS targets SYNM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name synemin, intermediate filament protein
Calculated molecular weight 173 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_015286
Gene Symbol SYNM
Gene ID (NCBI) 23336
RRIDAB_11042328
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15061
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYNM, also named as DMN, KIAA0353 and SYN, belongs to the intermediate filament family. SYNM is a Type-VI intermediate filament (IF) which plays an important cytoskeletal role within the muscle cell cytoskeleton. It forms heteropolymeric IFs with desmin and/or vimentin, and via its interaction with cytoskeletal proteins alpha-dystrobrevin, dystrophin, talin-1, utrophin and vinculin, is able to link these heteropolymeric IFs to adherens-type junctions, such as to the costameres, neuromuscular junctions, and myotendinous junctions within striated muscle cells. The antibody can't recognize isoform 2 or 3 of SYNM.