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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP (same clone as 30391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP (same clone as 30391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP (same clone as 30391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP (same clone as 30391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SYNJ2 Polyclonal antibody (30391-1-AP)

    IF Staining of U-251 using 30391-1-AP (same clone as 30391-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNJ2 antibody (30391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

SYNJ2 Polyclonal antibody, PBS Only

SYNJ2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30391-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30391-1-PBS targets SYNJ2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33002

Product name: Recombinant human SYNJ2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 678-839 aa of BC043277

Sequence: RRKKSAPAAFHLQVLQSNSQLLQGLTYNSSDSPSGHPPAAGTVFPQGDFLSTSSATSPDSDGTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSGTRSPKRDPIDPVSAGASAAKAELPPDHGHKTLGHWVTISDQEKRTALQVFDPLAKT

相同性解析による交差性が予測される生物種
Full Name synaptojanin 2
Calculated molecular weight 1496 aa, 166 kDa
Observed molecular weight143 kDa, 160 kDa
GenBank accession numberBC043277
Gene Symbol SYNJ2
Gene ID (NCBI) 8871
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15056
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

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