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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP (same clone as 30391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SYNJ2 Polyclonal antibody (30391-1-AP)

    WB analysis using 30391-1-AP (same clone as 30391-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP (same clone as 30391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using SYNJ2 Polyclonal antibody (30391-1-AP)

    IHC staining of mouse small intestine using 30391-1-AP (same clone as 30391-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using SYNJ2 Polyclonal antibody (30391-1-AP)

    IF Staining of U-251 using 30391-1-AP (same clone as 30391-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNJ2 antibody (30391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30391-1-PBS in a different storage buffer formulation.

SYNJ2 Polyclonal antibody, PBS Only

SYNJ2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30391-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30391-1-PBS targets SYNJ2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33002

Product name: Recombinant human SYNJ2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 678-839 aa of BC043277

Sequence: RRKKSAPAAFHLQVLQSNSQLLQGLTYNSSDSPSGHPPAAGTVFPQGDFLSTSSATSPDSDGTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSGTRSPKRDPIDPVSAGASAAKAELPPDHGHKTLGHWVTISDQEKRTALQVFDPLAKT

相同性解析による交差性が予測される生物種
Full Name synaptojanin 2
Calculated molecular weight 1496 aa, 166 kDa
Observed molecular weight143 kDa, 160 kDa
GenBank accession numberBC043277
Gene Symbol SYNJ2
Gene ID (NCBI) 8871
RRIDAB_3086305
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15056
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

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