Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, Jurkat cells |
| Positive IHC detected in | human brain tissue, mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
13893-1-AP targets Synaptojanin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4855 Product name: Recombinant human SYNJ2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1147-1496 aa of BC043277 Sequence: APQQPPKARTGISKPYNVKQIKTTNAQEAEAAIRCLLEARGGASEEALSAVAPRDLEASSEPEPTPGAAKPETPQAPPLLPRRPPPRVPAIKKPTLRRTGKPLSPEEQFEQQTVHFTIGPPETSVEAPPVVTAPRVPPVPKPRTFQPGKAAERPSHRKPASDEAPPGAGASVPPPLEAPPLVPKVPPRRKKSAPAAFHLQVLQSNSQLLQGLTYNSSDSPSGHPPAAGTVFPQGDFLSTSSATSPDSDGTKAMKPEAAPLLGDYQDPFWNLLHHPKLLNNTWLSKSSDPLDSGTRSPKRDPIDPVSAGASAAKAELPPDHGHKTLGHWVTISDQEKRTALQVFDPLAKT 相同性解析による交差性が予測される生物種 |
| Full Name | synaptojanin 2 |
| Calculated molecular weight | 1496 aa, 166 kDa |
| Observed molecular weight | 143, 160 kDa |
| GenBank accession number | BC043277 |
| Gene Symbol | SYNJ2 |
| Gene ID (NCBI) | 8871 |
| RRID | AB_2255784 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15056 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Synaptojanin 2 antibody 13893-1-AP | Download protocol |
| IHC protocol for Synaptojanin 2 antibody 13893-1-AP | Download protocol |
| WB protocol for Synaptojanin 2 antibody 13893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Metab Insulin signalling regulates Pink1 mRNA localization via modulation of AMPK activity to support PINK1 function in neurons | ||
Neuron Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy. | ||
Redox Biol Synaptojanin-2-binding protein ameliorates oxidative stress, neuroinflammation and depression-like behaviors via SYNJ2/PIP(2)/IP(3) signaling pathway. | ||
EMBO J A unified mechanism for mitochondrial damage sensing in PINK1-Parkin-mediated mitophagy. | ||
Front Cardiovasc Med Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis. | ||
Res Sq Bipolar and schizophrenia risk gene AKAP11 encodes an autophagy receptor coupling the regulation of PKA kinase network homeostasis to synaptic transmission |