Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
WB analysis using 24677-1-AP (same clone as 24677-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24677-1-AP (SYNJ1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)
IP result of anti-SYNJ1 (IP:24677-1-AP, 4ug; Detection:24677-1-AP 1:5000) with mouse brain tissue lysate 1960 ug. This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24677-1-AP (SYNJ1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IHC staining of human skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 24677-1-AP (SYNJ1 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.
IF Staining of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 24677-1-AP (SYNJ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24677-1-PBS in a different storage buffer formulation.

SYNJ1 Polyclonal antibody, PBS Only

SYNJ1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24677-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.1.3.36, INPP5G, KIAA0910, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1, synaptojanin 1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24677-1-PBS targets SYNJ1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20364 Product name: Recombinant human SYNJ1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 856-1125 aa of BC136603 Sequence: PVVALIDIDIFEVEAEERQNIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQQFASFGEVILIRFVEDKMWVTFLEGSSALNVLSLNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSAQSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPS 相同性解析による交差性が予測される生物種
Full Name	synaptojanin 1
Calculated molecular weight	1612 aa, 178 kDa
Observed molecular weight	140 kDa
GenBank accession number	BC136603
Gene Symbol	SYNJ1
Gene ID (NCBI)	8867
RRID	AB_2879668
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O43426
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Synaptojanin 1, a polyphosphoinositide phosphatase, is expressed as two major alternatively spliced isoforms of 145 kDa (SJ145) and 170 kDa (SJ170), which are thought to have pleiotropic roles in endocytosis, signaling, and actin function. SJ145 is highly enriched in nerve terminals where it participates in clathrin-dependent synaptic vesicle recycling. SJ170, which differs from SJ145 by the presence of a carboxy-terminal extension, is the predominant isoform in developing neurons and is expressed in a variety of tissues (PMID:10801423).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

WB analysis using 24677-1-AP (same clone as 24677-1-PBS)

WB analysis of NIH/3T3 using 24677-1-AP (same clone as 24677-1-PBS)

IP experiment of mouse brain using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of human brain using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of rat skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of rat brain using 24677-1-AP (same clone as 24677-1-PBS)

IHC staining of human skeletal muscle using 24677-1-AP (same clone as 24677-1-PBS)