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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SYNGR1 Monoclonal antibody

SYNGR1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H2H1

Cat no : 68176-1-Ig

Synonyms

synaptogyrin 1, SYNGR1

Validation Data Gallery

Applications:
  • WB analysis using 68176-1-Ig

    WB analysis using 68176-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of mouse retina using 68176-1-Ig

    WB analysis of mouse retina using 68176-1-Ig

    mouse retina tissue were subjected to SDS PAGE followed by western blot with 68176-1-Ig (SYNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68176-1-Ig

    IHC staining of rat brain using 68176-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68176-1-Ig (SYNGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68176-1-Ig

    IF Staining of mouse brain using 68176-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SYNGR1 antibody (68176-1-Ig, Clone: 2H2H1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inmouse retina tissue, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68176-1-Ig targets SYNGR1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SYNGR1 fusion protein Ag31309 相同性解析による交差性が予測される生物種
Full Name synaptogyrin 1
Calculated molecular weight 25 kDa
Observed molecular weight29 kDa
GenBank accession numberBC000731
Gene symbol SYNGR1
Gene ID (NCBI) 9145
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SYNGR1 antibody 68176-1-IgDownload protocol
IHC protocol for SYNGR1 antibody 68176-1-IgDownload protocol
IF protocol for SYNGR1 antibody 68176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols