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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SYNGR1 Polyclonal antibody (10432-1-AP)

    WB analysis using 10432-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10432-1-AP (SYNGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SYNGR1 Polyclonal antibody (10432-1-AP)

    IF Staining of A431 using 10432-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SYNGR1 antibody (10432-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SYNGR1 Polyclonal antibody

SYNGR1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10432-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Synaptogyrin-1, synaptogyrin 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, pig brain
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10432-1-AP targets SYNGR1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0685

Product name: Recombinant human SYNGR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-191 aa of BC000731

Sequence: MEGGAYGAGKAGGAFDPYTLVRQPHTILRVVSWLFSIVVFGSIVNEGYLNSASEGEEFCIYNRNPNACSYGVAVGVLAFLTCLLYLALDVYFPQISSVKDRKKAVLSDIGVSAFWAFLWFVGFCYLANQWQVSKPKDNPLNEGTDAARAAIAFSFFSIFTWSLTAALAVRRFKDLSFQEEYSTLFPASAQP

相同性解析による交差性が予測される生物種
Full Name synaptogyrin 1
Calculated molecular weight 25 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC000731
Gene Symbol SYNGR1
Gene ID (NCBI) 9145
RRIDAB_2200874
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43759
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

Protocols

Product Specific Protocols
WB protocol for SYNGR1 antibody 10432-1-APDownload protocol
IF protocol for SYNGR1 antibody 10432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols