Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
25151-1-AP targets Syncoilin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Syncoilin fusion protein Ag18565 相同性解析による交差性が予測される生物種 |
| Full Name | syncoilin, intermediate filament protein |
| Calculated molecular weight | 482 aa, 55 kDa |
| Observed molecular weight | 64-68 kDa, 55 kDa |
| GenBank accession number | BC119700 |
| Gene Symbol | SYNC |
| Gene ID (NCBI) | 81493 |
| RRID | AB_2879926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H7C4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syncoilin also known as SYNC1, is a member of the intermediate filament family with an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. Syncoilin is highly expressed in skeletal and cardiac muscle. In normal skeletal muscle, syncoilin is concentrated at the neuromuscular junction(PMID: 11053421). Western blot analysis detected 64 kDa and 55 kDa syncoilin proteins in mouse heart(PMID: 11053421).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syncoilin antibody 25151-1-AP | Download protocol |
| IHC protocol for Syncoilin antibody 25151-1-AP | Download protocol |
| IP protocol for Syncoilin antibody 25151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci The Cytoskeleton in the Extraocular Muscles of Desmin Knockout Mice. |