Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14565-1-AP targets SVOP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6134 Product name: Recombinant human SVOP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 261-548 aa of BC033587 Sequence: SARYDVLSGNQEKAIATLKRIATENGAPMPLGKLIISRQEDRGKMRDLFTPHFRWTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCGISSRKKAVEAKCSLACEYLSEEDYMDLLWTTLSEFPGVLVTLWIIDRLGRKKTMALCFVIFSFCSLLLFICVGRNVLTLLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGTCSGMARVGALITPFIAQVMLESSVYLTLAVYSGCCLLAALASCFLPIETKGRGLQESSHREWGQEMVGRGMHGAGVTRSNSGSQE 相同性解析による交差性が予測される生物種 |
| Full Name | SV2 related protein homolog (rat) |
| Calculated molecular weight | 61 kDa |
| Observed molecular weight | 67 kDa |
| GenBank accession number | BC033587 |
| Gene Symbol | SVOP |
| Gene ID (NCBI) | 55530 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N4V2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SVOP (Synaptic Vesicle 2-Related Protein) is an evolutionarily conserved, nonglycosylated transmembrane protein localized to synaptic vesicles and microvesicles in neurons and endocrine cells. Distantly related to SV2, it contains 12 transmembrane domains and shares homology with organic ion transporters. SVOP binds adenine nucleotides, particularly NAD, and is thought to contribute to synaptic vesicle function, though its precise physiological role remains to be fully elucidated. (PMID: 9801366)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SVOP antibody 14565-1-AP | Download protocol |
| WB protocol for SVOP antibody 14565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |