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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUZ12 Recombinant antibody (84895-3-RR)

    WB analysis using 84895-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84895-3-RR (SUZ12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SUZ12 Recombinant antibody (84895-3-RR)

    IF Staining of HeLa using 84895-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SUZ12 Recombinant antibody (84895-3-RR)

    IF Staining of MCF-7 using 84895-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SUZ12 antibody (84895-3-RR, Clone: 242491B3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using SUZ12 Recombinant antibody (84895-3-RR)

    FC experiment of HeLa using 84895-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SUZ12 Recombinant antibody (84895-3-RR, Clone:242491B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SUZ12 Recombinant monoclonal antibody

SUZ12 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84895-3-RR
Clone No.242491B3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

KIAA0160, Joined to JAZF1 protein, JJAZ1, CHET9, ChET 9 protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, COLO 320 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84895-3-RR targets SUZ12 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name suppressor of zeste 12 homolog (Drosophila)
Calculated molecular weight 83 kDa
Observed molecular weight80-90 kDa
GenBank accession numberNM_015355
Gene Symbol SUZ12
Gene ID (NCBI) 23512
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ15022
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUZ12, also termed CHET9, JJAZ1 or KIAA0160, belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family. It is a polycomb group (PcG) protein. SUZ12 is a component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. A chromosomal aberration involving SUZ12 may be a cause of endometrial stromal tumors.

Protocols

Product Specific Protocols
IF protocol for SUZ12 antibody 84895-3-RRDownload protocol
WB protocol for SUZ12 antibody 84895-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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