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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis using 10574-1-AP (same clone as 10574-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis of HepG2 using 10574-1-AP (same clone as 10574-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse small intestine tissue using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis of mouse small intestine using 10574-1-AP (same clone as 10574-1-PBS)

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis of HEK-293 using 10574-1-AP (same clone as 10574-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C6 cells using SUV39H1 Polyclonal antibody (10574-1-AP)

    WB analysis of C6 using 10574-1-AP (same clone as 10574-1-PBS)

    C6 cells were subjected to SDS PAGE followed by western blot with 10574-1-AP (SUV39H1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using SUV39H1 Polyclonal antibody (10574-1-AP)

    IP experiment of HeLa using 10574-1-AP (same clone as 10574-1-PBS)

    IP result of anti-SUV39H1 (IP:10574-1-AP, 4ug; Detection:10574-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 10574-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SUV39H1 Polyclonal antibody, PBS Only

SUV39H1 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 10574-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10574-1-PBS targets SUV39H1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0854

Product name: Recombinant human SUV39H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 7-277 aa of BC006238

Sequence: GCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVMEYVGEI

相同性解析による交差性が予測される生物種
Full Name suppressor of variegation 3-9 homolog 1 (Drosophila)
Calculated molecular weight 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC006238
Gene Symbol SUV39H1
Gene ID (NCBI) 6839
RRIDAB_2196710
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43463
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.

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