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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SURF2 Polyclonal antibody

SURF2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16522-1-AP

Synonyms

SURF 2, SURF2, surfeit 2, Surfeit locus protein 2

Validation Data Gallery

Applications:
  • WB analysis using 16522-1-AP

    WB analysis using 16522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of BxPC-3 using 16522-1-AP

    WB analysis of BxPC-3 using 16522-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 16522-1-AP

    WB analysis of COLO 320 using 16522-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16522-1-AP

    WB analysis of HeLa using 16522-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16522-1-AP (SURF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 16522-1-AP

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16522-1-AP

    IHC staining of human breast cancer using 16522-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16522-1-AP (SURF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 16522-1-AP

    IF Staining of HeLa using 16522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16522-1-AP (SURF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 16522-1-AP

    IF Staining of HeLa using 16522-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16522-1-AP (SURF2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, HeLa cells, COLO 320 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16522-1-AP targets SURF2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SURF2 fusion protein Ag9745 相同性解析による交差性が予測される生物種
Full Name surfeit 2
Calculated molecular weight 256 aa, 30 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC014411
Gene symbol SURF2
Gene ID (NCBI) 6835
RRIDAB_2255415
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SURF2 antibody 16522-1-APDownload protocol
IHC protocol for SURF2 antibody 16522-1-APDownload protocol
IF protocol for SURF2 antibody 16522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols