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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SURF1 Recombinant antibody

SURF1 Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230210E12

Cat no : 83052-6-RR

Synonyms

SURF 1, SURF1, surfeit 1, Surfeit locus protein 1

Validation Data Gallery

Applications:
  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83052-6-RR

    WB analysis using 83052-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83052-6-RR (SURF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 83052-6-RR

    FC experiment of HepG2 using 83052-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SURF1 (83052-6-RR, Clone:230210E12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, mouse liver tissue, MCF-7 cells, rat liver tissue, rat heart tissue
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

83052-6-RR targets SURF1 in WB, FC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SURF1 fusion protein Ag3959 相同性解析による交差性が予測される生物種
Full Name surfeit 1
Calculated molecular weight 33 kDa
Observed molecular weight30-31 kDa
GenBank accession numberBC028314
Gene symbol SURF1
Gene ID (NCBI) 6834
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SURF1 antibody 83052-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols