Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, rat kidney tissue, HEK-293 cells, PC-3 cells, NIH/3T3 cells, 3T3-L1 cells, mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86706-1-RR targets SUPV3L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3542 Product name: Recombinant human SUPV3L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 441-786 aa of BC036112 Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD 相同性解析による交差性が予測される生物種 |
| Full Name | suppressor of var1, 3-like 1 (S. cerevisiae) |
| Calculated molecular weight | 786 aa, 88 kDa |
| Observed molecular weight | 70-80 kDa |
| GenBank accession number | BC036112 |
| Gene Symbol | SUPV3L1 |
| Gene ID (NCBI) | 6832 |
| RRID | AB_3745068 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IYB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SUPV3L1 antibody 86706-1-RR | Download protocol |
| WB protocol for SUPV3L1 antibody 86706-1-RR | Download protocol |
| IF protocol for SUPV3L1 antibody 86706-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |