Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, Jurkat cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
27556-1-AP targets SUN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SUN2 fusion protein Ag16077 相同性解析による交差性が予測される生物種 |
| Full Name | unc-84 homolog B (C. elegans) |
| Calculated molecular weight | 717 aa, 80 kDa |
| Observed molecular weight | 80-85 kDa |
| GenBank accession number | BC111717 |
| Gene Symbol | SUN2 |
| Gene ID (NCBI) | 25777 |
| RRID | AB_2880906 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UH99 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SUN2, also known as UNC84B, RAB5IP, belongs to the SUN (Sad1 and UNC84) protein family, which is a group of conserved proteins in eukaryotes. SUN2 is a key component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, which connects the nuclear lamina to the cytoskeleton. This connection is crucial for the transmission of mechanical forces across the nuclear envelope and for nuclear movement and positioning (PMID: 18396275).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SUN2 antibody 27556-1-AP | Download protocol |
| IHC protocol for SUN2 antibody 27556-1-AP | Download protocol |
| IF protocol for SUN2 antibody 27556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Tumor cells impair immunological synapse formation via central nervous system-enriched metabolite | ||
Virology Long non-coding RNA SUN2-AS1 acts as a negative regulator of ISGs transcription to promote flavivirus infection |