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Validation Data Gallery

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  • Sandwich ELISA sample test of MP03279-2

    Sandwich ELISA sample test of MP03279-2

    The mean SUMO1 concentration was determined to be 19.73 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load, 33.97 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 21.00 ng/mL in NIH/3T3 cell extract based on a 1.50 mg/mL extract load.

  • Sandwich ELISA standard curve of MP03279-2

    Sandwich ELISA standard curve of MP03279-2

    Sandwich ELISA standard curve of MP03279-2, Human SUMO1 Recombinant Matched Antibody Pair - PBS only. 87942-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0414. 87942-2-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

  • Cytometric bead array sample test of MP03279-1

    Cytometric bead array sample test of MP03279-1

    The mean SUMO1 concentration was determined to be 75.1 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 76.0 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP03279-1

    Cytometric bead array standard curve of MP03279-1

    Cytometric bead array standard curve of MP03279-1, SUMO1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 87942-3-PBS. Detection antibody: 87942-2-PBS. Standard: Ag0414. Range: 0.312-40 ng/mL.

SUMO1 Recombinant monoclonal antibody, PBS Only (Detector)

SUMO1 Uni-rAb® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 87942-2-PBS
Clone No.253578D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

87942-2-PBS targets SUMO1 as part of a matched antibody pair:

MP03279-1: 87942-3-PBS capture and 87942-2-PBS detection (validated in Cytometric bead array)

MP03279-2: 87942-4-PBS capture and 87942-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0414

Product name: Recombinant human SUMO1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-101 aa of BC006462

Sequence: MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV

相同性解析による交差性が予測される生物種
Full Name SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Calculated molecular weight 12 kDa
GenBank accession numberBC006462
Gene Symbol SUMO1
Gene ID (NCBI) 7341
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP63165
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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