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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULT2B1 Polyclonal antibody (29185-1-AP)

    WB analysis using 29185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skin tissue using SULT2B1 Polyclonal antibody (29185-1-AP)

    WB analysis of mouse skin using 29185-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 29185-1-AP (SULT2B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29185-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, mouse skin tissue, LNCaP cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29185-1-AP targets SULT2B1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULT2B1 fusion protein Ag30209 相同性解析による交差性が予測される生物種
Full Name sulfotransferase family, cytosolic, 2B, member 1
Calculated molecular weight 365 aa, 41 kDa
Observed molecular weight41 kDa
GenBank accession numberBC034694
Gene Symbol SULT2B1
Gene ID (NCBI) 6820
RRIDAB_2918245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate.

Protocols

Product Specific Protocols
WB protocol for SULT2B1 antibody 29185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols