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Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    WB analysis of human lung using 12522-1-AP (same clone as 12522-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    WB analysis of human liver using 12522-1-AP (same clone as 12522-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human adrenal gland tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    WB analysis of human adrenal gland using 12522-1-AP (same clone as 12522-1-PBS)

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    WB analysis of mouse kidney using 12522-1-AP (same clone as 12522-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    WB analysis of human kidney using 12522-1-AP (same clone as 12522-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12522-1-AP (SULT1E1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    IP experiment of mouse liver using 12522-1-AP (same clone as 12522-1-PBS)

    IP result of anti-SULT1E1 (IP:12522-1-AP, 4ug; Detection:12522-1-AP 1:400) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SULT1E1 Polyclonal antibody (12522-1-AP)

    IHC staining of human breast cancer using 12522-1-AP (same clone as 12522-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12522-1-AP (SULT1E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12522-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SULT1E1 Polyclonal antibody, PBS Only

SULT1E1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12522-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12522-1-PBS targets SULT1E1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3216

Product name: Recombinant human SULT1E1 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-294 aa of BC027956

Sequence: MNSELDYYEKFEEVHGILMYKDFVKYWDNVEAFQARPDDLVIATYPKSGTTWVSEIVYMIYKEGDVEKCKEDVIFNRIPFLECRKENLMNGVKQLDEMNSPRIVKTHLPPELLPASFWEKDCKIIYLCRNAKDVAVSFYYFFLMVAGHPNPGSLPEFVEKFMQGQVPYGSWYKHVKSWWEKGKSPRVLFLFYEDLKEDIRKEVIKLIHFLERKPSEELVDRIIHHTSFQEMKNNPSTNYTTLPDEIMNQKLSPFMRKGITGDWKNHFTVALNEKFDKHYEQQMKESTLKFRTEI

相同性解析による交差性が予測される生物種
Full Name sulfotransferase family 1E, estrogen-preferring, member 1
Calculated molecular weight 294 aa, 35 kDa
Observed molecular weight 32-35 kDa
GenBank accession numberBC027956
Gene Symbol SULT1E1
Gene ID (NCBI) 6783
RRIDAB_2302772
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49888
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SULT1E1, also known as estrogen sulfotransferase, catalyzes the sulfation of endogenous estrogens as well as xenobiotic estrogen-like chemicals, converting them into the inactive form. SULT1E1 is a 33-35 kDa cytosolic protein that has been detected in human hepatic and nonhepatic tissues (17035602). SULT1E1 is expressed in breast and endometrial tissues, and some studies have investigated its implication in tumor development. Higher expression of SULT1E1 was observed in breast cancer tissues compared with normal breast tissues (22380844). This antibody detected a major band around 35 kD in lysates of mouse kidney, human liver and so on.

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