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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis using 16050-1-AP (same clone as 16050-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis of human liver using 16050-1-AP (same clone as 16050-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human colon tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    WB analysis of human colon using 16050-1-AP (same clone as 16050-1-PBS)

    human colon tissue were subjected to SDS PAGE followed by western blot with 16050-1-AP (SULT1B1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human liver cancer using 16050-1-AP (same clone as 16050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human colon cancer using 16050-1-AP (same clone as 16050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SULT1B1 Polyclonal antibody (16050-1-AP)

    IHC staining of human colon cancer using 16050-1-AP (same clone as 16050-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16050-1-AP (SULT1B1 antibody) at dilution of 1:240 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16050-1-PBS in a different storage buffer formulation.

SULT1B1 Polyclonal antibody, PBS Only

SULT1B1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16050-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

SULT1B2, ST1B2, ST1B1, EC:2.8.2.1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16050-1-PBS targets SULT1B1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9009

Product name: Recombinant human SULT1B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-296 aa of BC010895

Sequence: MLSPKDILRKDLKLVHGYPMTCAFASNWEKIEQFHSRPDDIVIATYPKSGTTWVSEIIDMILNDGDIEKCKRGFITEKVPMLEMTLPGLRTSGIEQLEKNPSPRIVKTHLPTDLLPKSFWENNCKMIYLARNAKDVSVSYYHFDLMNNLQPFPGTWEEYLEKFLTGKVAYGSWFTHVKNWWKRKEEHPILFLYYEDMKENPKEEIKKIIRFLEKNLNDEILDRIIHHTSFEVMKDNPLVNYTHLPTTVMDHSKSPFMRKGTAGDWKNYFTVAQNEKFDAIYETEMSKTALQFRTEI

相同性解析による交差性が予測される生物種
Full Name sulfotransferase family, cytosolic, 1B, member 1
Calculated molecular weight 296 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC010895
Gene Symbol SULT1B1
Gene ID (NCBI) 27284
RRIDAB_2302767
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43704
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Sulfotransferase 1B1 (SULT1B1) is expressed at highest levels throughout the human colon and small intestine but can also be found at moderate levels in human liver, kidney, and white blood cells (PMID: 28084139). Certain SULT1B1 SNPs may influence the activity of thyroid hormones and the mutagenicity of polycyclic aromatic hydrocarbons (PMID: 34107842).

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