Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 19499-1-AP (same clone as 19499-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of Caco-2 using 19499-1-AP (same clone as 19499-1-PBS)
Caco-2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 19499-1-AP (same clone as 19499-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 19499-1-AP (same clone as 19499-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 19499-1-AP (same clone as 19499-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
WB analysis of HuH-7 using 19499-1-AP (same clone as 19499-1-PBS)
HuH-7 cells were subjected to SDS PAGE followed by western blot with 19499-1-AP (SULT1A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IP experiment of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)
IP result of anti-SULT1A3 (IP:19499-1-AP, 4ug; Detection:19499-1-AP 1:200) with COLO 320 cells lysate 2240ug. This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer using 19499-1-AP (SULT1A3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19499-1-AP (SULT1A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19499-1-PBS in a different storage buffer formulation.

SULT1A3 Polyclonal antibody, PBS Only

SULT1A3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 19499-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Sulfotransferase 1A3/1A4, SULT1 A3, SULT1A 3, SULT1A4

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19499-1-PBS targets SULT1A3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	sulfotransferase family, cytosolic, 1A, phenol-preferring, member 3
Calculated molecular weight	34 kDa
Observed molecular weight	30-34 kDa
GenBank accession number	NM_177552
Gene Symbol	SULT1A3
Gene ID (NCBI)	6818
RRID	AB_10642560
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P0DMM9
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

SULT1A3, also named as STM and SULT1A4, belongs to the sulfotransferase 1 family. SULT1A3 catalyzes the sulfate conjugation of phenolic monoamines (neurotransmitters such as dopamine, norepinephrine and serotonin) and phenolic and catechol drugs.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of Caco-2 using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of MCF-7 using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of HepG2 using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of HT-29 using 19499-1-AP (same clone as 19499-1-PBS)

WB analysis of HuH-7 using 19499-1-AP (same clone as 19499-1-PBS)

IP experiment of COLO 320 using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human hepatocirrhosis using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)

IHC staining of human breast cancer using 19499-1-AP (same clone as 19499-1-PBS)

SULT1A3 Polyclonal antibody, PBS Only

SULT1A3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Background Information

Able™

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