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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using SULF2 Polyclonal antibody (12260-1-AP)

    WB analysis of mouse liver using 12260-1-AP (same clone as 12260-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SULF2 Polyclonal antibody (12260-1-AP)

    WB analysis using 12260-1-AP (same clone as 12260-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12260-1-AP (SULF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SULF2 Polyclonal antibody (12260-1-AP)

    IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using SULF2 Polyclonal antibody (12260-1-AP)

    IHC staining of mouse ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using SULF2 Polyclonal antibody (12260-1-AP)

    IHC staining of mouse heart using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12260-1-AP (SULF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using SULF2 Polyclonal antibody (12260-1-AP)

    IHC staining of human ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using SULF2 Polyclonal antibody (12260-1-AP)

    IHC staining of human ovary using 12260-1-AP (same clone as 12260-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 12260-1-AP (SULF2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12260-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

SULF2 Polyclonal antibody, PBS Only

SULF2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12260-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Arylsulfatase, EC:3.1.6.1, EC:3.1.6.14, Extracellular sulfatase Sulf 2, Extracellular sulfatase Sulf-2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12260-1-PBS targets SULF2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2915

Product name: Recombinant human SULF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 722-870 aa of BC020962

Sequence: MPGLTCFTHDNQHWQTAPFWTLGPFCACTSANNNTYWCMRTINETHNFLFCEFATGFLEYFDLNTDPYQLMNAVNTLDRDVLNQLHVQLMELRSCKGYKQCNPRTRNMDLGLKDGGSYEQYRQFQRRKWPEMKRPSSKSLGQLWEGWEG

相同性解析による交差性が予測される生物種
Full Name sulfatase 2
Calculated molecular weight 870 aa, 96 kDa
Observed molecular weight 50 kDa, 130-150 kDa
GenBank accession numberBC020962
Gene Symbol SULF2
Gene ID (NCBI) 55959
RRIDAB_11183762
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IWU5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SULF2, also named as KIAA1247, belongs to the sulfatase family. It exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. SULF2 can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. SULF2 has several forms: the unglycosylated 100 kDa SULF2, the glycosylated 120-150 kDa SULF2, the cleaved 72-75 kDa and 50 kDa SULF2.

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