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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using SUDS3 Polyclonal antibody (25845-1-AP)

    WB analysis of Raji using 25845-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 25845-1-AP (SUDS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using SUDS3 Polyclonal antibody (25845-1-AP)

    WB analysis of HL-60 using 25845-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 25845-1-AP (SUDS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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SUDS3 Polyclonal antibody

SUDS3 Polyclonal Antibody for WB, ELISA
Cat No. 25845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SAP45, SDS3, SUDS3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25845-1-AP targets SUDS3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SUDS3 fusion protein Ag22961 相同性解析による交差性が予測される生物種
Full Name suppressor of defective silencing 3 homolog (S. cerevisiae)
Calculated molecular weight 328 aa, 38 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC112000
Gene Symbol SUDS3
Gene ID (NCBI) 64426
RRIDAB_2880265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H7L9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SUDS3 (Sin3 histone deacetylase corepressor complex component SDS3), also known as SAP45 andSDS3, is a component of the Sin3 histone deacetylase corepressor complex. In this complex SDS3 interacts with SIN3A to regulate gene expression via chromatin modification. SUDS3 represses transcription and augments histone deacetylase activity of HDAC1. It may have a potential role in tumor suppressor pathways through regulation of apoptosis.

Protocols

Product Specific Protocols
WB protocol for SUDS3 antibody 25845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols